Nceda uzenzele olwakho ukhetho ngokusekwe kwimeko yelabhoratri yakho.
Kubalulekile ukufumana indlela esebenzayo yokucoca i-vials yesampuli
Okwangoku, kukho inani elikhulu leesampuli zeemveliso zezolimo (ezinye iimveliso zeekhemikhali, i-organic acids, njl.) ezifuna ukuvavanywa nge-chromatography yamanzi kunye ne-chromatography yegesi minyaka yonke.Ngenxa yenani elikhulu leisampulu, kukho inani elikhulu leebhotile zesampulu ezifuna ukucocwa ngexesha lenkqubo yokubhaqwa, engachithi nje ixesha kwaye icuthe ukusebenza kakuhle, kodwa ngamanye amaxesha ibangela ukutenxa kwiziphumo zovavanyo ngenxa yokucoceka. iibhotile zesampuli ezicociweyo.
Iibhotile zesampulu zechromatographic zenziwe ngeglasi, kunqabile ukuba zenziwe ngeplastiki.Iingqayi zesampulu ezilahlwayo zibiza imali eninzi, ziyinkcitho, kwaye zingcolisa okusingqongileyo.Iilabhoratri ezininzi zicoca iingqayi zesampulu kwaye ziphinde zisetyenziswe.
Okwangoku, iindlela ezixhaphakileyo zaselabhoratri zokuhlamba iingqayi zongezwa isicoci, isicoci, izinyibilikisi eziphilayo kunye nokuhlamba iasidi, kwaye emva koko inkqubo yetyhubhu encinci yokubrasha esisigxina.
Le ndlela yesiqhelo yokukhuhla inezinto ezininzi ezingalunganga:
Ukusetyenziswa kwe-detergent kudla amanzi amaninzi, ixesha lokuhlamba lide, kwaye kukho iikona ezingenakucoceka.Ukuba ngaba yisampuli yeplastiki yeebhotile, kulula ukuba namanqaku ebrashi ngaphakathi kodonga lweebhotile, ezithatha izibonelelo zabasebenzi ezininzi.Kwi-glassware engcoliswe kakhulu yi-lipid kunye ne-protein residues, isisombululo se-alkaline lysis sisetyenziselwa ukucoca, kwaye iziphumo ezilungileyo zifumaneka.
Xa uhlalutya iisampuli nge-LC / MS / MS, ukucocwa kwe-injection vials kubaluleke kakhulu.Ngokwendlela yokucoca i-glassware, indlela yokucoca ikhethwe ngokwezinga lokungcola.Akukho ndlela imiselweyo.Indlela isishwankathelo:
Ukhetho Lokuqala:
1. Thela isisombululo sovavanyo kwi-vials ezomileyo
2. Gcoba sonke isisombululo sovavanyo kwi-95% yotywala, uhlambe kabini nge-ultrasonic kwaye uyithulule, kuba utywala bungena lula kwi-vial ye-1.5mL kwaye inokuxutywa kunye ne-solvents ezininzi ze-organic ukuze ufezekise umphumo wokucoca.
3. Thela emanzini acocekileyo, kwaye uhlambe i-ultrasonically kabini.
4. Thela i-lotion kwi-vials eyomileyo kwaye ubhake kwi-110 degrees Celsius ngeeyure ezi-1 ukuya kwezi-2.Ungaze ubhake kwiqondo lokushisa eliphezulu.
5. Pholisa kwaye ugcine.
Ukhetho lwesibini:
1. Hlanza ngamanzi ompompo amaxesha amaninzi
2. Yifake kwibeaker ezaliswe ngamanzi acocekileyo (umatshini wamanzi acocekileyo we-Millipore) kunye ne-sonicate imizuzu eyi-15
3. Guqula amanzi kunye ne-ultrasound imizuzu eyi-15
4. Gxila kwibhika ezaliswe yi-ethanol ngokupheleleyo (iQela leSinopharm, uHlahlelo olusulungekileyo)
5. Ekugqibeleni, yikhuphe kwaye uyeke umoya wome.
Ukhetho lwesithathu:
1. Okokuqala gcoba kwi-methanol (i-chromatographically pure), kwaye i-ultrasonically icoceke imizuzu engama-20, uze uthele i-methanol eyomileyo.
2. Gcwalisa iibhotile zesampula ngamanzi, kwaye ucoceke nge-ultrasonically imizuzu engama-20, uthele amanzi .
3. Yomisa iibhotile zesampuli emva koko.
Ukhetho lwesine:
Indlela yokuhlamba i-vials yesampula iyafana nokulungiswa kwesigaba solwelo, njl njl. Okokuqala, sebenzisa utywala lwezonyango ukucwina iiyure ezingaphezu kwe-4, emva koko i-ultrasound isiqingatha seyure, emva koko ugalele utywala lwezonyango, kwaye usebenzise amanzi. kwisiqingatha se-ultrasound.Iiyure, hlambulula ngamanzi kwaye uyomise.
Ukhetho lwesihlanu:
Okokuqala, gcoba kwisisombululo esinamandla sokucoca i-oxidizing (i-potassium dichromate) kwiiyure ezingama-24, uze usebenzise amanzi adibeneyo kwi-ultrasonic Geza kathathu phantsi kweemeko, kwaye ekugqibeleni uhlambe nge-methanol kanye, uze uyomise ukuze uyisebenzise.
I-caps septas kufuneka itshintshwe, ngakumbi xa uhlalutya iintsalela ze-pesticide, ngaphandle koko kuya kuchaphazela iziphumo zobungakanani.
Kodwa ukuba iimeko zivuma, zama ukusebenzisa izinto ezilahlayo, ezifana nokufakwa kwe-PTFE okulahlwayo okanye ukufakwa kweplastiki yasekhaya (malunga ne-0.1 yuan / iqhekeza), kunye neebhotile zesampuli zilungile.Ukusetyenziswa okuphindaphindiweyo kwaye akufuneki ukuba kucocwe.
Ukhetho lwesithandathu:
(1) nzima inkqubo yokucoca eneziphumo eziphathekayo:
No1.Emva kokuba kusetyenziswe iibhotile zesampuli, hlambulula iibhotile zesampuli ngamanzi asebenzayo kuqala, kwaye uhlambulule isampuli eseleyo (unokuyigungqa ngesandla ngexesha elifanayo);
No2, emva koko faka iisampulu zeengqayi kwi-potassium dichromate yokuhlamba i-bubble yolwelo, kwaye xa iqokelela Xa ufikelela kwisixa esithile okanye xa ukwimo elungileyo, yikhuphe kwitanki yokuthambisa kwaye uyibeke kwisefu yeplastiki ekhitshini. sebenzisa.Hlanza kakuhle ngamanzi epompo.Unokuhlunga ngokuphindaphindiweyo kwaye ugubungele phakathi;
No3.Sebenzisa amanzi aphuma empompo ukuze ucoce i-ultrasonically amaxesha ama-3 emva kokuhlanjululwa.Ngokujikelezileyo, kungcono ukugubha amanzi kwiibhotile zesampuli emva kokucoca kwe-ultrasonic nganye;
I-No4, ke usebenzise amanzi ahlanjululweyo kathathu (okanye amanzi ahlambulukileyo, amanzi adibeneyo) kunye ne-1.3 yokucoca i-ultrasonic kathathu;
No5, emva koko sebenzisa i-chromatographic ecocekileyo ye-methanol yokucoca i-ultrasonic amaxesha angama-2-3, kungcono kakhulu
vuthulula i-methanol kwiibhotile zesampuli emva kokucoca ngalunye;
No6.Beka iisampulu zesampulu kwi-oven kwaye uyomise malunga ne-80 degrees, kwaye ingasetyenziswa.
(2) iisampulu zeengqayi ezithengiweyo ukuze ziphawule ngemibala eyahlukeneyo:
ukuba uye waqaphela ukuba kukho uphawu oluncinci olunombala kwisampulu yeengqayi, engeyiyo inkangeleko entle, kodwa inokusetyenziswa.Xa uthenga, kungcono ukuthenga iibhotile ezininzi zemibala eyahlukeneyo.
Umzekelo: ilabhoratri yakho ivula iiprojekthi ezimbini A no-B ngaxeshanye.Ngexesha lokuqala iprojekthi isebenzisa iibhotile zesampuli ezimhlophe, kunye neprojekthi ye-B isebenzisa isampuli eluhlaza okwesibhakabhaka.Emva kokuba uvavanyo lugqityiwe, lucocwa ngokwendlela engentla, kunye novavanyo lwesibini Ngelo xesha, sebenzisa iibhotile zesampula eluhlaza okwesibhakabhaka kwiprojekthi ye-A, iibhotile zesampuli ezimhlophe zeprojekthi ye-B, njalo njalo, enokuphepha ngokufanelekileyo ingxaki ebangelwa ungcoliseko lomsebenzi wakho.
Bhala ekugqibeleni
1. Iinjineli ezininzi zezixhobo ziye zacebisa: Sebenzisa isithando somlilo kwi-400 degrees ukubhaka isiqingatha seyure, izinto eziphilayo ziphelile;
2. Beka iisampulu yeengqayi kwisithando somlilo ukuze yomiswe ngamaqondo angama-300 ngokukaCelsius.Injineli ye-Agilent evela eBeijing yathi xa ifika kwisithando somlilo, uvavanyo aluyi kuba nomsindo emva kokubhaka kwisithando somlilo kwi-300 degrees kwiiyure ze-6.
kwakhona …………..
Iiflasks ezincinci zevolumetric, iiflasks ezimile okwepere zomoya ojikelezayo, kunye nezinye izixhobo zeglasi zokuhlalutya okanye ukunyangwa kwangaphambili zinokucocwa ngokubhekiselele kule ndlela.
Ixesha lokuposa: Feb-25-2022