Iinaliti zegesi zechromatographsebenzisa ngokubanzi 1ul kunye 10ul. Nangona inaliti yokutofa incinci, iyafuneka. Inaliti yokutofa ngumjelo odibanisa isampuli kunye nesixhobo sokuhlalutya. Ngenaliti yenaliti, isampuli ingangena kwikholamu yechromatographic kwaye idlule kwi-detector yohlalutyo oluqhubekayo lwe-spectrum. Ngoko ke, ukugcinwa kunye nokucocwa kwenaliti yenaliti kugxininiso lwengqwalasela yemihla ngemihla yabahlalutyi. Ngaphandle koko, akuyi kuchaphazela kuphela ukusebenza kakuhle, kodwa kubangele nomonakalo kwisixhobo. Lo mfanekiso ulandelayo ubonisa amacandelo enaliti yokutofa.
Ukuhlelwa kweenaliti zenaliti
Ngokwenkangeleko yenaliti yenaliti, inokwahlulwa ibe ziinaliti zenaliti ezicolisiweyo, iinaliti zenaliti ye-bevel, kunye neenaliti ezisicaba. Iinaliti zeConical zisetyenziselwa inaliti ye-septum, enokunciphisa umonakalo kwi-septum kwaye imelane neenaliti ezininzi. Zisetyenziswa ikakhulu kwii-injection ezizenzekelayo; iinaliti ze-bevel zingasetyenziswa kwi-injection septa, ekulula ukuyisebenzisa. Phakathi kwazo, iinaliti ze-26s-22 zifaneleke kakhulu ukusetyenziswa kwi-injection septa kwi-chromatography yegesi; iinaliti ezinentloko-esicaba zisetyenziswa ikakhulu kwiivalvu zesitofu kunye neesampulu zeepipette zekhromatographs zolwelo ezisebenza kakhulu.
Ngokwendlela yokutofa, inokwahlulwa ibe yinaliti yokutofa ezenzekelayo kunye nenaliti yezandla.
Ngokweemfuno zokuhlalutya ezahlukeneyo zenaliti yokutofa kwi-chromatograph yegesi kunye nolwelo lwechromatograph engamanzi, inokwahlulwa ibe yinaliti yokutofa kwerhasi kunye nenaliti yenaliti yolwelo. Inaliti yerhasi yechromatography ifuna inaliti encinci, kwaye owona mthamo uxhaphakileyo wenaliti yi-0.2-1ul, ke inaliti ehambelanayo nenaliti ngokubanzi yi-10-25ul. Inaliti ekhethiweyo yinaliti yohlobo lwe-cone, olulungele ukusebenza kwesitofu; Xa kuthelekiswa, umthamo wenaliti yolwelo we-chromatography bukhulu ngokubanzi, kwaye umthamo wenaliti eqhelekileyo yi-0.5-20ul, ngoko ke umthamo wenaliti ohambelana nawo mkhulu, ngokubanzi ngama-25-100UL, kwaye incam yenaliti ithe tyaba ukunqanda ukukrwela i-stator.
Kucazululo lwechromatographic, inaliti yesitofu esiqhele ukusetyenziswa yinaliti encinci, elungele ngokukodwa ichromatograph yegesi kunye nohlalutyo lwe-chromatograph engamanzi. Iyonke impazamo yomthamo wayo yi-±5%. Ukusebenza kwe-airtight ukumelana ne-0.2Mpa. Yahlulwe yaba ziindidi ezimbini: isitofu sokugcina ulwelo kunye nesitofu sokugcina ulwelo. Uluhlu oluchaziweyo lwe-non-liquid micro-injector yi-0.5μL-5μL, kunye noluhlu lwenkcazo ye-micro-injector engamanzi yi-10μL-100μL. Inaliti encinci yokutofa sisixhobo esichanekileyo esiyimfuneko.
Ukusetyenziswa kwe-injector
(1) Jonga isitofu phambi kokuba uyisebenzise, khangela ukuba isirinji inazo na iintanda kunye nokuba incam yenaliti igqabhukile na.
(2) Susa isampuli eshiyekileyo kwi-injector, hlamba i-injector nge-solvent 5 ~ 20 amaxesha, kwaye ulahle ulwelo lwenkunkuma ukusuka kumaxesha angama-2 ~ 3 okuqala.
(3) Susa amaqamza kwi-injector, cwilisa inaliti kwi-solvent, kwaye ngokuphindaphindiweyo udwebe isampuli. Xa ukhupha isampuli, iibhubhu kwi-injector zinokutshintsha ngokutshintsha ngokuthe nkqo kwetyhubhu.
(4) Xa usebenzisa i-injector, qala ngokugcwalisa i-injector ngolwelo, uze emva koko ukhuphe ulwelo kumthamo ofunekayo wokutofa.
Ukugcinwa kwenaliti yokutofa
(1) Iisampuli ze-viscosity eziphakathi ukuya eziphezulu kufuneka zihlanjululwe okanye inaliti enkulu yangaphakathi yenaliti kufuneka ikhethwe ngaphambi kokusetyenziswa.
(2) Xa kucocwa inaliti, kufuneka kusetyenziswe izixhobo zokucoca, ezinjengocingo olukhokelayo okanye i-stylet, i-tweezers, ne-surfactants ukucoca udonga lwenaliti.
(3) Ukucoca i-Thermal: Ukucoca i-thermal kusetyenziselwa ukususa i-organic residues kwinaliti, ngakumbi uhlalutyo lomkhondo, indawo yokubilisa ephezulu kunye nezinto ezincamathelayo. Emva kwemizuzu embalwa yokucoca i-thermal, isixhobo sokucoca inaliti singasetyenziswa kwakhona.
Ukucoca inaliti yokutofa
1. Udonga lwangaphakathi lwenaliti yenaliti lunokucocwa nge-solvent ephilayo. Xa ucoca, nceda ujonge ukuba inaliti yokutyhala inaliti ingahamba kakuhle;
2. Ukuba inaliti yokutyhala inaliti ayihambi kakuhle, intonga yokutyhala inokususwa. Kucetyiswa ukuba uyisule ngelaphu elithambileyo elifakwe kwi-solvent ephilayo.
3. Sebenzisa ngokuphindaphindiweyo i-solvent ye-organic ukunqwenela. Ukuba ukuchasana nenaliti yokutyhala ngenaliti yonyuka ngokukhawuleza emva kweminqweno emininzi, oko kuthetha ukuba kusekho ukungcola okuncinci. Kule meko, inkqubo yokucoca kufuneka iphindwe.
4. Ukuba inaliti yokutyhala inaliti inokuhamba kakuhle kwaye ngokuthe chu, khangela ukuba inaliti ivalekile na. Uhlambulule ngokuphindaphindiweyo inaliti nge-solvent ephilayo kwaye ujonge imilo yesampulu etyhalwa ngaphandle kwenaliti.
5. Ukuba inaliti yokutofa iqhelekile, isampuli iya kuphuma ngomgca othe ngqo. Ukuba inaliti ivaliwe, isampuli iya kutshizwa kwinkungu ecolekileyo ukusuka kwicala elinye okanye i-angle. Nangona i-solvent ngamanye amaxesha iphuma kumgca othe tye, qaphela ukuba ukhangele ukuba ukuhamba kungcono kunesiqhelo (thelekisa nje ukuhamba kunye nenaliti entsha, engavinjelwanga yenaliti).
6. Ukuvinjelwa kwinaliti kuya kutshabalalisa ukuveliswa kwakhona kohlalutyo. Ngenxa yesi sizathu, ukugcinwa kwenaliti kuyimfuneko. Sebenzisa into efana nocingo ukususa ukuvaleka kwinaliti. Inaliti ingasetyenziselwa kuphela xa isampuli iphuma ngokuqhelekileyo. Ukusebenzisa i-pipette kwi-aspirate liquid okanye isicoci sesirinji sinokususa ngokufanelekileyo ukungcola kwinaliti.
Izilumkiso xa usebenzisa inaliti yokutofa
Musa ukubamba inaliti yesirinji kunye nenxalenye yesampulu ngezandla zakho, kwaye ungabi namaqamza (xa unqwenela, khupha kancinci, ngokukhawuleza, uze ufune ngokukhawuleza, phinda amaxesha amaninzi, 10 Umthamo wenaliti yesinyithi yesirinji ye-μl yi-0.6 Ukuba kukho amaqamza, awunowabona ngaphezulu kwe-1-2μl kwaye ujongise incam yenaliti phezulu de amaqamza aye phezulu, emva koko utyhale intonga yenaliti ukususa amaqamza. i-syringe ene-core ivakalelwa kukuba i-flat) Isantya senaliti kufuneka sikhawuleze (kodwa singakhawulezi kakhulu), gcina isantya esifanayo kwisitofu ngasinye, kwaye uqale ukutofa isampuli xa incam yenaliti ifikelela kumbindi wegumbi lokufunxa.
Indlela yokuthintela inaliti yokutofa ekugobeni? Abaninzi abaqalayo abenza uhlalutyo lwechromatography bahlala begoba inaliti kunye nentonga yesirinji yesirinji. Izizathu zezi:
1. Indawo yokutofa ikrwelwe kakhulu. Ukuba i-screwed kakhulu kwiqondo lokushisa lokushisa, isitywina se-silicone siya kwandisa kwaye siqinise xa ubushushu begumbi lokufunxa bukhuphuka. Ngeli xesha, kunzima ukufaka isirinji.
2. Inaliti inamathele kwinxalenye yentsimbi ye-injection port xa isikhundla singafumaneki kakuhle.
3. Intonga yesirinji igobile ngenxa yokuba kusetyenziswa amandla amaninzi ngexesha lokutofa. I-chromatographs eyoyikekayo, evela kumazwe angaphandle iza kunye ne-injector rack, kwaye i-injection nge-injector rack ayiyi kugoba intonga yesirinji.
4. Ngenxa yokuba udonga lwangaphakathi lwesirinji lungcolisekile, intonga yenaliti ityhalwa kwaye igotywe ngexesha lokutofa. Emva kokusebenzisa isirinji ixesha elithile, uya kufumana into encinci emnyama kufuphi nomphezulu wetyhubhu yenaliti, kwaye kuya kuba nzima ukumunca isampuli kunye nokutofa. Indlela yokucoca: Khupha intonga yenaliti, ugalele amanzi amancinci, faka intonga yenaliti kwindawo engcolileyo kwaye utyhale kwaye utsale ngokuphindaphindiweyo. Ukuba ayisebenzi kube kanye, tofa amanzi kwakhona de isingcolisi sisuswe. Ngeli xesha, uya kubona ukuba amanzi akwisirinji ayaxokozela. Khupha intonga yenaliti kwaye uyisule ngephepha lokucoca, kwaye emva koko uyihlambe ngotywala amaxesha amaninzi. Xa isampuli eza kuhlalutywa yisampuli eqinileyo echithwe kwi-solvent, hlamba isirinji nge-solvent ngexesha emva kokutofa.
5. Qinisekisa ukuba uzinzile xa utofa. Ukuba unqwenela ukukhawuleza, isirinji iya kugoba. Ngethuba nje unobuchule kwinaliti, iya kukhawuleza.
Ixesha lokuposa: Jun-19-2024